Platypus Paradox

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The platypus puzzles naturalists and scientists alike. While its bizarre characteristics seem to defy a natural explanation, the platypus may be a classic transitional link. Like a reptile, it lays eggs, yet, it nurses with milk without nipples. As one of the least understood living mammals, and unlike any other known species, it has ten sex chromosomes. The platypus produces venom, like a reptile, and uses electroreception, like a shark – a puzzle known as the platypus paradox dilemma.

That’s not all; the list of oddities goes on. To gain an understanding of this evolution icon, scientists have long-awaited insights from its genome. This January’s journal Nature reports on the most comprehensive investigation of the platypus genome ever performed.

Genome Findings

Entitled “Platypus and Echidna Genomes Reveal Mammalian Biology and Evolution,” the report generated immediate and widespread media coverage on nature’s only two known egg-laying mammals.

In the New York Times article entitled “A Question Hidden in the platypus Genome: Are We the Weird Ones?” writer and researcher Cara Giaimo (pictured right) gives a snapshot intro for the platypus paradox story –

“When the British zoologist George Shaw (his platypus drawing pictured right) below first encountered a platypus specimen in 1799, he was so befuddled that he checked for stitches, thinking someone might be trying to trick him with a Franken creature. It’s hard to blame him: What other animal has a rubbery bill, ankle spikes full of venom, luxurious fur that glows under black light, and a tendency to lay eggs?”

Alex Fox, a science journalist, writing for the Smithsonian Magazine in the article entitled “Platypus Genes Are Just as Odd as the Creature Itself,” explains the significance of the report –

“The platypus genome was first sequenced in 2008… [however], the quality and completeness of this new genome analysis is a massive improvement…. The new genome is 96 percent mapped, meaning individual genes are in their proper locations on the chromosomes, compared to 25 percent in 2008.”

The author of January’s report, Yang Zhou (pictured left) of Beijing Genomics Institute in China, summarized the goal of the report –

“The new genomes of both species will enable further insights into their innovations and the biology and evolution of these extraordinary egg-laying mammals.”

As the only two species in the Monotremes order, the investigators studied both platypus and echidna genomes. Evolution geneticists anticipated finding the most similarities between these two species.

Connecting Transitional Species

Charles Darwin envisioned natural selection acting through “slight successive” changes, which he sometimes called “gradations.” At other times, he referred to these as “intermediate gradations,” “Intermediate forms,”

“intermediate varieties,” “intermediate links,” “intermediate states,” and so on, as well as “transitional links” – a new version of Aristotle’s “Great Chain of Being.”

Platypus by George Shaw, 1799

As such, the assortment of platypus oddities seemed to validate his concept; gradations are a natural product of evolution. The number of these connecting species in the Great Chain, Darwin argued, was predicted as “innumerable” –

“The number of intermediate and transitional links, between all living and extinct species, must have been inconceivably great.”

In the late eighteenth century, stories about the platypus paradox began generating widespread curiosity – long before Darwin. The president of the Royal Society, Sir Joseph Banks (1743-1820), wrote to English naturalist George Caley (1770-1829), living in Australia at the time, requesting –

“What I want to know is the mode of generation [evolution] of the Duck Bill [platypus] and the Porcupine anteater [echidna].”

Naturalists were puzzled about how to classify these odd quirks of nature. Nevertheless, French naturalist Etienne Geoffroy St-Hilaire (1772-1844) (pictured left) was the first to propose classifying the platypus and echidna as mammals – coining the term “monotreme.” As Ann Moyal explains in ”Platypus, the extraordinary story of how a curious creature baffled the world,” explains the daunting new challenge –

“The long struggle to give a taxonomic definition and position of the elusive platypus had begun.”

Jean Baptiste Lamarck (1744-1829), a French naturalist, objected to the mammal classification since neither species had mammary glands. In his Philosophie Zoologique, Lamarck developed a different classification scheme for these two species.

Darwin’s focus, however, centered on understanding the mechanisms of evolution – not on classification. In the Origin of Species, Darwin viewed these two as transitional species –

“… in freshwater, we find some of the most anomalous forms now known in the world, as the Ornithorhynchus [platypus] and Lepidosiren, which, like fossils, connect to a certain extent [living] orders at present widely sundered in the natural scale [Great Chain of Being].”

The idea of connecting species on Aristotle’s “chain” was not unique to Darwin. Six centuries earlier, St. Albertus Magnus (1200–1280), a German Catholic (pictured right), maintained –

“Nature does not make [animal] kinds separate without making something intermediate between them; for nature does not pass from extreme to extreme without an intermediate.”

For Darwin, the platypus seemed like an evolutionary intermediate, even more than the Archaeopteryx. While mentioning the Archaeopteryx only two times in the Origin of Species, Darwin mentions the platypus seven times. For example, in using a tree of life approach, Darwin wrote –

“As we here and there see a thin straggling branch springing from a fork low down in a tree … we occasionally see an animal like the Ornithorhynchus [platypus] and Lepidosiren [Lung Fish], which in some small degree connects [transitionally] by its affinities two large branches of life.”

Even Darwin (pictured left), however, recognized a problem with transitional links – the number of known species with gradations are not “innumerable.” In the Origin of Species, Darwin concedes –

“The distinctness of specific forms and their not being blended together by innumerable transitional links is a very obvious difficulty.”

The fossil record challenge to identify “transitional links” continues today – the problem is even on display in the current Smithsonian Human Evolution exhibit.

For these reasons, scientists have increasingly turned to molecular biology for evidence of intermediate forms – starting with proteomics in the mid-twentieth century. By the beginning of the twenty-first century, however, genomics has emerged as the preferred method. Optimism has increasingly soared.

First Genome Study, 2008

The first platypus genome report entitled the “Genome Analysis of the Platypus Reveals Unique Signatures of Evolution” appeared in the journal Nature (2008). Using one female platypus genome, Wesley C. Warren (pictured right), a geneticist at the Washington University School of Medicine, organized a research team from thirty-two institutions to investigate, analyze and report on the platypus genome.

While not finding evidence for Darwin’s “slight successive” gradations in the genome, the team interestingly found the platypus genome to contain a similar number of protein-coding genes found in other mammals.

Remarkably, while approximately 18,500 coding genes of the platypus genome were found to have more than 80 percent of its genes shared with a wide range of distant species, no transitional links were identified. In conclusion, Warren noted –

“We find that the mixture of reptilian, mammalian, and unique characteristics of the platypus genome provides many clues to the function and evolution of all mammalian genomes.”

Warren’s report, however, mentions intermediate forms only once – without any species identified as an intermediate or transitional.

Likewise, Adam Felsenfeld of the NIH Comparative and Sequencing Analysis Program summarized Warren’s report by not referencing any intermediate or transitional forms. In the report published in NIH’s “News Releases” entitled “Duck-Billed Platypus Genome Sequence Published, Animal’s Reptilian-Mammalian Mix Reflected in Its DNA, ” conclude –

“This genome provides a unique perspective on what the genomes of our earliest mammalian ancestors may have looked like. It is fascinating that what we think of as being reptile-like and mammal-like features can co-exist in the same genome.”

The report explains the phylogenetics of the platypus paradox – reptile features stem from reptilian genes, and mammalian features have mammalian genes. Significantly, however, the report found no molecular evidence for Darwin’s “innumerable” gradations. Does the emperor seem to have no clothes?

In reviewing the 2008 study, co-leader Frank Grutzner of the University of Adelaide noted –

“This finding revolutionized our understanding of mammalian sex chromosome evolution but also raised fundamental new questions.”

The “new questions” draw the concept of transitional links into question.

2021 Study Goals

Geneticists, since 2008, have increasingly uncovered more of nature’s hidden secrets by using speedier, more precise, and advanced genetic sequencing technologies.

Earlier genomic testing, by comparison, was rudimentary, limited, and sometimes erroneous. In comparison to the 2008 study, Zhou notes –

“Only about 25% of its sequence was [earlier] assigned to chromosomes… [in this study, there was] a 1,390-fold improvement for the contig N50 and a 49-fold improvement for the scaffold N50… We performed extensive error correction… Ambiguous chromosome assignments were resolved.”

For this comprehensive project, Zhou organized a forty-membered team of world-renowned experts in bioinformatics, cytogenetics, and molecular biology, including scientists from Australia, China, Japan, Denmark, and the USA for the task. To focus on transitional links, the team studied the platypus and the echidna, along with a wide range of distant species.

“In my opinion, among mammals, the platypus is the most fascinating species of all,” Warren added. “They represent the ancestral state of what terrestrial mammal genomes could have been before adapting to various environments.”

However, the platypus even differs from the echidna in many aspects. For example, the platypus is a carnivore, while the echidna is an insectivore; the platypus uses electroreception-orientation to navigate, while the echidna uses olfaction-orientation to navigate.

Given how these two monotremes are similar yet distinctive, they are “well-suited for the study of evolution,” notes Zhou. Yet, as Zhou explains –

“The incomplete platypus assembly [from the 2008 study] without Y chromosome sequences [only a female studied] and lack of an echidna genome have limited the interpretation of the evolution of mammals and monotremes.”

Scientists have long speculated on the biomolecular mechanisms driving the platypus oddities. Zhou’s report received comprehensive media coverage. Liam Shaw, writing for the London Review, noted –

“Last week, the first near-complete platypus genome was published. This is an impressive achievement.”

From the new evidence observed in the genomes, in the words of Alex Fox (pictured right), writing for the Smithsonian Magazine, however, signaled the new dilemma –

“Platypus genes are just as odd as the creature Itself.”

2021 Study Findings

A comprehensive biological account is essential to understand the molecular mechanisms driving evolution. While the 2008 platypus genome report was informative, it was inaccurate. As Zhou notes –

“Complete and accurate reference genomes and annotations are critical for evolutionary and functional analyses.”

Zhou’s team updated the female genome and sequenced and mapped the male platypus and echidna genome (pictured left). By comparing these two genomes and comparing these with a wide range of distant species, the team gained new insights into the long-standing platypus paradox dilemma– for the first time. The study concluded by noting –

“We delineate ancient and lineage-specific changes in the sensory system, haemoglobin degradation, and reproduction that represent some of the most fascinating biology of platypus and echidna.”

The team’s findings validate previous assumptions by “adding much more clarity,” said Wesley Warren, pointing to the distinctive nature of the platypus. As Cara Giaimo reported in The New York Times –

“By diving into their DNA, researchers can uncover the genes and proteins that underpin some of these creatures’ distinctive traits.”

However, as in Warren’s report, Zhou report never identified any species as an intermediate or transitional species. Giamimo’s article concluded by noting –

“Dr. Zhang [co-author] and his colleagues plan to continue investigating the many monotreme mysteries that remain. ‘They are a very important lineage to understand,’ [Warren] said.”

The mystery continues. Centuries after George Shaw encountered the platypus paradox; scientists are still searching for a natural explanation to solve the platypus paradox dilemma. Not surprisingly, scientific evidence from the genomic revolution continues to undermine long-standing icons of evolution.

In one sentence, WIKIPEDIA’s evolution section on the Platypus page understates the new dilemma with Zhou’s new study by simply stating –

“An updated genome, the most complete on record, was published in 2021, together with the genome of the short-beaked echidna.”

The following Appendix outlines the list of molecular oddities reported in Zhou’s study.

Genesis

The most recent and advanced genome investigation ever performed only further complicates the long-standing evolution dilemma – the platypus has no transitional links as predicted by the theory of evolution. Long-standing speculations on evolution are increasingly challenged by the genomic revolution. Instead of a transitional link, Zhou’s study undermines Darwin’s theory that the Platypus is a transitional or intermediate link or gradation.

Since the platypus is biologically distinctive without any genomic links or gradations with any species studied, the evidence is compatible with Moses‘s Genesis record.

Sir Isaac Newton, an English physicist, and mathematician, recognized as one of the most influential scientists of all time and a key figure in the scientific revolution, explains the problem with explaining life without an intelligent Creator during the Scientific Revolution –

“How came the Bodies of Animals to be contrived with so much Art, and for what ends were their several parts? Was the Eye contrived without Skill in Opticks, and the Ear without Knowledge of Sounds? … And these things being rightly dispatch’d, does it not appear from Phaenomena that there is a Being incorporeal, living, intelligent omnipresent…”

Evidence from the fields of anatomy and morphology to validate the theory of evolution scientifically still remains speculative.

Click to study evolutionary perspectives on anatomy and morphology plus other crucial Evolution and Science categories further.

Darwin Then and Now is an educational resource on the intersection of evolution and science and the challenges facing the theory of evolution.

Appendix

Key findings by category from Zhou’s study published in the journal Nature, 2021

Diet Adaptations

Of eight genes involved in tooth development [in mammals], four genes were lost in both monotreme genomes.
Echidnas (but not platypuses) further lost two enamel genes
Loss of digestive genes (reported in platypus) is shared with Echidna
NGN3—essential for stomach and pancreas development—has been maintained in both species
Chemosensory – eutherian [human] mammals have more than 25 copies of bitter taste receptor genes (TAS2R genes), whereas this gene family is considerably smaller in monotremes with only 7 in platypus.

Hemoglobin Adaptations

Both the haemopexin and alpha-1 microglobulin genes are found in the monotreme genomes, whereas the haptoglobin gene in monotremes
Haptoglobin evolved in the common ancestor of vertebrates from an immune gene of the MASP family but has neofunctionalized in mammals to bind to hemoglobin with a higher affinity and to bind to the CD163A receptor, which is also absent in monotremes, for clearance in macrophages
monotremes lack genes that cluster with haptoglobin in the MASP family or a PIT54 orthologue
expansion of the CD163 family in the platypus (ten members) and found five in Echidna, compared with two and three in humans and mice

Transition from oviparity to viviparity

Whereas reptiles have three functional copies of the significant egg protein vitellogenin (VTG)38, in monotremes, we found only one functional copy (VTG2)
SPINT3, a major milk-specific protein present in early lactation of therians with a probable role in the protection of immunoincompetent young in marsupials, is absent in monotremes.
The monotreme genomes contain most of the milk genes that have been identified in therian mammals. Most mammals have three casein genes
In addition to these genes, monotremes have extra caseins that are not found in therian mammals, with unknown functions, an extra copy of CSN2 (CSN2B) and CSN3 (CSN3B) in the platypus, which has the classic structure of CSN342.
Extant monotremes appear to have lost both ODAM and FDCSP – secretory calcium-binding phosphoprotein (SCPP) gene [fond in mammals.

Conclusion

Without evidence of any genetic transitional links, as once predicted by the theory of evolution, phylogenic evidence points to the platypus’s uniqueness as a distinctive species.